Ea. hy926 cells
WebFigure 1 The effect of piperlongumine on the viability of EA.hy926 (A) and A549 (B) cells. Notes: The cells were treated with different concentrations of piperlongumine (1–8 μM) for 24 hours and cell viability was assessed by MTT colorimetric assay. Data are expressed as a percentage of the untreated cells (control). Statistically significant difference at P<0.05 … WebDec 9, 2024 · EA.hy926 cells were cultured in DMEM/F12 medium with 5% fetal calf serum, 0.5 µm aminopterin, 16 µm thymidine, and 100 µm hypoxanthine. Experiments …
Ea. hy926 cells
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WebMar 15, 2024 · EA.hy926 cells express very little RNA for caspase-5 and inflammasome sensors, NLRP3 and NLRC4, that are essential for caspase-1 activation . We stimulated … WebTo cite this cell line use: EA.hy 926 (RRID:CVCL_3901) Comments Group: Space-flown cell line (cellonaut). Characteristics: Produced by the fusion of A549/8 with human umbilical vein endothelial cells. Doubling time: 12 hours (PubMed= 6407019 ). Omics: Transcriptome analysis by microarray.
WebSep 2, 2024 · The EA.hy926 cells, derived from the fusion of human umbilical vein endothelial cells (HUVECs) with a thioguanine-resistant clone of A549, were used as the … WebMay 15, 2024 · EA.hy926 endothelial cells were seeded at 9000 cells/cm 2 on 96 well plates pre-coated with Matrigel (5 μL Matrigel mixed in 95 μL DMEM) or without Matrigel (Panel A) or Collagen Type I dissolved in acetic acid at concentrations of 6 and 10 μg/cm 2 (Panel B) and grown for up to 10 days without changing the media. Cell number was …
WebOct 31, 2024 · EA.hy926 endothelial cells were seeded into a 96-well plate at the density of 3 × 104 cells per well with various concentrations of leptin (0, 0.1, 1, 5, 10, or 50 ng/ml). … WebDec 18, 2024 · Before You Begin EA.hy926 Cell Culture. Timing: 2 days. 1. We have generated EA.hy926 cells (ATCC) stably expressing pLenti-blasticidin-GFP or pLenti …
WebOct 31, 2024 · EA.hy926 endothelial cells were seeded into a 96-well plate at the density of 3 × 104 cells per well with various concentrations of leptin (0, 0.1, 1, 5, 10, or 50 ng/ml). Premixed CCK-8 (10 μl) and medium were …
WebPurpose: The low dose radiation response of primary human umbilical vein endothelial cells (HUVEC) and its immortalized derivative, the EA.hy926 cell line, was evaluated and … slow cookers with auto settingWebJul 27, 2024 · Human umbilical vein endothelial cells (HUVECs, Ea.hy926) were used to observe the cells adhesion to the samples. Firstly, the sterilized samples were put into 24-well culture plates, then the endothelial cells were seeded on the samples under a concentration of 2 × 10 4 cells/mL. After a 24 h incubation period, the samples were … slow cookers with domed lidsWebJun 3, 2016 · (E) EA.hy926 cells were stimulated with individual human recombinant histone (20 μg/mL H1, H2A/H2B, H3.3, H4) for 4 h, and the expression of surface TF antigens was determined by flow cytometry. All data are presented as mean ± SEM. The data were combined data from 3 or more different experiments. soft tissue nodule in left hemipelvisWebMar 1, 1996 · EA.hy926 cells were found to be more responsive than HUVECs to thrombin, as a lower concentration of the agonist was necessary to induce maximum monolayer permeability. Responses of HUVECs and EA.hy926 cells to thrombin were consistent with a pathway involving the thrombin receptor. A thrombin mutant in which the active-site … soft tissue neck x-ray sidWebFigure 3 Smyd1 up-regulates and activates nuclear translocation of NF-kappaB in EA.hy926 cells. ( A ) Representative flow cytometry histograms of EA.hy926 cells transfected with a pCMV2-Smyd1-flag vector or a vector without gene insert (control) and incubated with a monoclonal antibody recognizing the p65 subunit of NF-kappaB and, subsequently ... slow cookers with stainless steel insertsWebEA.hy926 cell line (7), which mai- n tains an extensively differentiated ph-e notype (20), was also found to be r- e fractory to transfection, despite its vigorous growth properties. After opt-i mization of 5 transfection methods for EA.hy926 cells, Teifel et al. (23) found that the best transfection efficiency achieved was 2%. soft tissue neck x-ray positioningWebApr 13, 2024 · EA.hy926 cells within the logarithmic growth phase were seeded in 96-well plates (1 × 10 4 cells/cm 2) and incubated with different concentrations of phenolic acid (1, 5, or 25 μM) for 1 h. They were then separately co-incubated with TNF-α (10 ng/mL) or without TNF-α for 24 h. ELISA kits (Abcam, Cambridge, UK) were used to determine the ... soft tissue neck x ray sid